How to annotate clusters.
A good starting point for cluster annotation is to transfer labels from an existing dataset or external reference. To do so, click the label transfer toggle and select a dataset to transfer labels from:
Change cluster name
To manually change a cluster name toggle the rename cluster button:
Then type a new name for the cluster and click the rename cluster button:
Sorting marker genes
Selecting a cluster will sort marker genes for that cluster:
To compare the selected cluster to one other cluster, click the toggle single group comparisons button and select a comparison:
Comparing two clusters is particularly useful for distinguishing closely related clusters and for confirming doublet clusters.
Exploring marker genes
Selecting a gene will plot its logcounts in cells and clusters:
External gene data
Click the BioGPS toggle button to view the relative expression of the selected gene in the groups from the BioGPS dataset: